What is FISH?

Fluorescence in situ hybridization (FISH) is a sensitive and accurate technique that enables the detection of chromosomal aberrations and is complementary to conventional cytogenetic analysis. The method entails hybridization of a single-stranded fluorescently labeled nucleic acid sequence (probe), which is complementary to a target genomic sequence that is present in metaphase chromosomes as well as interphase nuclei and is able to detect the presence or absence of a given abnormality.

The primary advantage of the FISH technique is its applicability to non-dividing cells and a variety of specimen types. FISH is a preferred method for diagnosis, prognosis, treatment response, and minimal residual disease detection in a variety of hematopoietic neoplasms and solid tumors.

FISH Overview

Fluorescence in situ hybridization (FISH) Steps

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