Four Color, Enumeration Probe

Ref: 25-002

Several screening programs for cervical cancer have been successfully implemented in the clinical community, and are comprised of tests based on the appearance of abnormal cells in cytological specimens, and more recently, based on HPV type. There remains, however, a great need for additional biomarkers that will increase the sensitivity with which precancerous and cancerous lesions are detected. Furthermore, there is a lack of testing available for the identification of atypical or moderately abnormal squamous cell lesions that will progress to higher grades.

The FISH-based HPV-Associated Cancer Test (FHACTTM) is the only four color FISH Probe that can be used for cervical cancer screening as additional triage before referral for colposcopy. FHACTTM can be used on leftover thin prep specimen (no resampling) and conventional Pap smears.

 FHACT drawing

Fluorescence in situ hybridization (FISH) is a molecular cytogenetics technique that permits visualization and mapping of specific genes or loci on metaphase chromosomes or interphase nuclei. FHACTTM is a Probe designed to determine copy number changes of the 3q26 (TERC, 5p15 (D5S2095), 20q13 (D20S911), and Cen7 regions by FISH.

In normal diploid interphase and metaphase cells, FHACTTM generates two red, two green, two blue and two gold signals corresponding to the normal chromosomes 3, 5, 7 and 20 respectively. In cells with gain or amplification of 3q26, 5p15, 20q13 and/or chromosome 7, the number of red, green, gold and/or blue signals will be greater than 2 each.

FHACTTM is protected by the following patents:
Methods for Detecting Human Papilloma Virus-Associated Cancers, US, 13/474,111 (Sept. 6, 2012) and PCT/US2011/050681 (March 15, 2012)

If you want to view our webinar about FHACTTM , click here.



FHACTTM is a trademark of Cancer Genetics, Inc.

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